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The use of molecular techniques for - AVHANDLINGAR.SE

The PCR-SSP technique in the blood group diagnostic field is the method of choice for a low amount of samples and/or for confirmation tests. By means of sequence specific primers (SSP) only determined sequences in the examined gene are amplified highlighting different mutations in the gene sequence. The PCR-RFLP technique requires 4 steps: DNA extraction, amplification, restriction enzyme digestion and electrophoresis. The time required for the performance of the technique was 19 h30 min.

Pcr ssp technique

DNA extraction. 22. SSR markers. 22. PCR. 23 and Cross-Species Amplification in.

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Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Advances in molecular biology techniques allowed for introduction of PCR-based methods for HLA typing.

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[ Links ] 47 Parasannanavar DJ, Rajadhyaksha A, Ghosh K. Application of a simple in-house PCR-SSP technique for HLA-B* 27 typing in spondyloarthritis patients. Arthritis. HLA typing by sequence-specific oligonucleotides probes (PCR-SSOP) Example of hybridization specificity with SSO probes. The combination of PCR technology and hybridization with sequence-specific oligonucleotide probes was first applied to HLA class II typing because of the limitations of DR serology and of the better knowledge of allelic polymorphism at DR/DQ loci. pcr-sspを行う場合，プライマーをどの位置に設定するかということが重要なポイントになってくる．プライマーは3'末端部位に検出したい配列がくるように設計する．pcr-ssp法は3'末端が全く同じプライマーであれば，pcr増幅が行われるが，3'末端が異なっていると，その部位でプライマーが相補 Discrepant HLA typing results were detected in 6 of 33 samples (18.2%) analyzed by two different PCR-SSO techniques and one PCR-SSP technique. The first sample was typed as HLA-A*24, A*30 by PCR-SSP and as HLA-A*24, A*32 by PCR-SSO . A real-time reverse-transcriptase PCR (RT-PCR) technique was developed for the rapid and specific detection and enumeration of viable Alternaria spp.

Ourmain aimwas to standardize a simple inexpensive in-house PCR-SSP technique forHLA-B ∗ 27 typing.Materials andMethods. HLA Typing Technologies.
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sativa This project aims to validate the techniques in a local context together with farmers, Simultaneously, we also developed Kompetitive Allele Specific PCR (KASP) Dekoko (Pisum sativum ssp. abyssinicum) to enhance its contribution to food This technique has potential to become a biomarker for treatment decisions, and to The SSP was combined with a gene fusion detection module (analyzing ALK, and Shc1 activations were studied using real-time PCR and Western blots.

PCR. 23 and Cross-Species Amplification in. P. ssp.. Crop Science 42, 2128-2136. av J Thelaus · Citerat av 1 — One of the main goals of this collaboration is harmonisation of methods and har ett stort fokus lagts på utveckling av molekylär diagnostik, med realtids PCR på följande odlingsplattor: Y. pestis på blodagar bas (BAB), F. tularensis ssp.
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P. brassicae hanus sativus ssp. oleifera were tested for.